Felivet FPV-FHV-FCV Ab Combo Test
Short Introduction
The Felivet FPV-FHV-FCV Ab Combo Test is a lateral flow immunochromatographic assay for the semi-quantitative detection of FPV-FHV-FCV Antibody (FPV-FHV-FCV Ab) in cat's serum, or plasma.
Assay Time: 10 minutes
Specimen: serum or plasma
Principle
The Felivet FPV-FHV-FCV Ab Combo Test is based on sandwich lateral flow immunochromatographic assay. The test card has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated FPV-FHV-FCV recombinant antigens. If there are FPV-FHV-FCV antibodies in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can indicate the presence of FPV-FHV-FCV antibodies in the specimen.
Test Procedure
-Allow all materials, including specimen and test device, recover to 15-25ºC before running the assay.
-Take out the test device from the foil pouch and place it horizontally.
-Drop 1 drops (approx. 40μL)of the serum or plasma specimen into the sample hole "S" of the test device then drop two drops(approx. 80μL) of buffer.
-Interpret the result in 10 minutes. Result after 15 minutes is considered as invalid.
-The sequence of the T line is shown in the figure.
Interpretation Of Result
The presence of "C" line indicates a valid result. Read the result by comparing the color intensity of the "T" line with the standard color reference scale (from "G1" to "G6").
The titers of FPV-FHV-FCV antibodies are corresponding with the color intensity grades.
| G1 < 1:20 |
G2 = 1:20 |
G3 = 1:40 |
| G4 = 1:80 |
G5 = 1:160 |
G6 > 1:320 |
-Positive (+): The presence of both "C" line and zone "T" line. T line at G3 is considered as standard positive. T line < G3, is weak positive and indicates low FPV-FHV-FCV antibody level. T line around G4-G5, is strong positive. T line > G6, is very strong positive, indicates a contact with virus recently or in infection.
-Negative (-): Clear C line appears. No T line, or T line is weaker than G1. It indicates very low FPV-FHV-FCV antibody titer. Failed immunization may happen.
-Invalid: No colored line appears in C zone.
Certification
Company Profile
Hangzhou Evegen Biotech Co., Ltd. has a top colloidal gold product research and development team, strong technical strength, product quality ahead of similar products at home and abroad, after several times with the domestic and foreign well-known veterinary diagnostic reagents team cooperation, development and launch a series of broad market potential of livestock and poultry and pets with rapid diagnostic test paper products.
With the positive attitude and concept of win-win, the company develops more and better products for the international veterinary rapid diagnosis field. We are willing to contribute our youth and wisdom to the agriculture and animal disease detection industry. We are willing to join hands with those who are interested in creating the spring of rapid diagnosis of animal diseases in China!
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