Ovine Brucella Antibody Rapid Test
Short Introduction
Ovine brucellosis is a reproductive disease that can affect all breeds of sheep. The disease may cause economic loss in flocks by reducing lambing percentages, extending lambing seasons and increasing ram culling.
It is a disease primarily of rams. Ewes usually only carry the infection for one or two oestrus cycles.In rams the disease causes inflammation of the epididymis. In pregnant ewes, B. ovis causes inflammation of the placenta.
The Ovine Brucella Antibody Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of ovine Brucella Antibody (O.Brucella Ab) in sheep's serum or plasma specimen.
Principle
The Ovine Brucella Antibody Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated Brucella LPS antigens. If there are anti-Brucella antibodies in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Ovine Brucella antibodies in the specimen.
Test Procedure
-Collect sheep's fresh whole blood, serum or plasma specimen for use. Immediately apply the testing once collected the specimen.
-Take out the test device from the foil pouch and place it horizontally.
-Place 1 drop (approx. 20μL) of serum or plasma specimen into an assay buffer tube. Mix well and directly use in the assay.
-Place 2-3 drops of assay buffer into the sample hole of the test card.
-Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
Interpretation Of Result
- Positive (+): The presence of both "C" line and zone "T" line, no matter T line is clear or vague.
- Negative (-): Only clear C line appear. No T line.
- Invalid: No colored line appears in C zone. No matter if T line appears.
Certification
Company Profile
Hangzhou Evegen Biotech Co., Ltd. has a top colloidal gold product research and development team, strong technical strength, product quality ahead of similar products at home and abroad, after several times with the domestic and foreign well-known veterinary diagnostic reagents team cooperation, development and launch a series of broad market potential of livestock and poultry and pets with rapid diagnostic test paper products.
With the positive attitude and concept of win-win, the company develops more and better products for the international veterinary rapid diagnosis field. We are willing to contribute our youth and wisdom to the agriculture and animal disease detection industry. We are willing to join hands with those who are interested in creating the spring of rapid diagnosis of animal diseases in China!
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