Canine Rabies Virus Antibody Quantitative Test
Short Introduction
Rabies is a vital disease to both animal and human beings. Dog or cat could be a carrier without any clinical symptom. Vaccination could be apparently decrease the ratio of carrier and infection of rabies virus. But even after vaccination, there is still about 15% of total cases which could not generate protective antibodies. It is suggested to detect the Rabies antibodies for dogs after 45 days vaccination.
The Canivet Rabies Ab Quanti-Test is a lateral flow immunochromatographic assay for the quantitative detection of rabies virus antibody (Rabies Ab) above the level 0.5 IU/mL in dog's serum or plasma.
Detection Range: 0.5 IU/mL~25 IU/mL
Assay Time: 10 minutes
Assay Specimen: Serum or plasma
Principle
The Canivet Rabies Ab Quanti-Test is based on sandwich method lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay.
When the treated specimen was applied into the specimen hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated recombinant rabies antigen. If the rabies virus antibody (Rabies Ab) level is above 0.5 IU/mL in the specimen, a visible T line will appear and the results of rabies antibodies level could be obtained by the RDT-Calculator. The C line should always appear after the specimen is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of rabies antibodies in the specimen.
Test Procedure
Allow all reagents and materials, including specimen and test device, to balance at 15-25ºC for 30 minutes before running the assay.
-Take out the test device from the foil pouch and place it horizontally. Do not pick up the device from the platform until the testing is completed.
-Adjust the pipette scale to suck 10 μL specimen and add it into the specimen hole "S" of the test device.
-Replace the pipette tip. Adjust the pipette scale to suck 90 μL assay buffer and add it into the specimen hole "S". Start the timer.
(Note: pipette tips used for serum/plasma specimen should not be used for assay buffer to prevent contamination of assay buffer).
-Interpret the result in 10 minutes by using the RDT-Calculator. Reading time at 9 to 11 minutes is acceptable. Result out of this range is not credible for quantitative test. Result after 15 minutes is considered as invalid.
Interpretation Of Result
- Positive (+): The presence of both "C" line and zone "T" line, no matter T line is clear or vague.
- Negative (-): Only clear C line appear. No T line.
- Invalid: No colored line appears in C zone. No matter if T line appears.
Certification
Company Profile
Hangzhou Evegen Biotech Co., Ltd. has a top colloidal gold product research and development team, strong technical strength, product quality ahead of similar products at home and abroad, after several times with the domestic and foreign well-known veterinary diagnostic reagents team cooperation, development and launch a series of broad market potential of livestock and poultry and pets with rapid diagnostic test paper products.
With the positive attitude and concept of win-win, the company develops more and better products for the international veterinary rapid diagnosis field. We are willing to contribute our youth and wisdom to the agriculture and animal disease detection industry. We are willing to join hands with those who are interested in creating the spring of rapid diagnosis of animal diseases in China!
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