Ovine Foot and Mouth Disease Virus Non-Structural Protein 3ABC Antibody Test
Short Introduction
Foot-and-mouth disease (FMD) is an acute, febrile and highly contagious disease common to even-toed ungulates. Its clinical features are blisters and ulcers in the oral mucosa, hoof and breast of the affected animal.
The Foot and Mouth Disease Virus Anti-O Antibody Rapid Test (FMDV-O Ab) is a lateral flow immunochromatographic assay for the qualitative detection of serotype-O FMDV antibodies in pig's serum, plasma. VP1 protein recombinant was applied in this assay, for the monitoring of FMDV-O antibody level and providing an important reference for the vaccination plan.
Principle
The FMD NSP Ab Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated FMDV 3ABC antigens. If there are anti-3ABC antibodies in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of FMDV 3ABC antibodies in the specimen.
Test Procedure
-Collect sheep's fresh whole blood, and get the serum or plasma specimen for use. Immediately apply the testing once collected the specimen.
-Take out the test device from the foil pouch and place it horizontally.
-Place 3 drops (around 120uL) of serum or plasma specimen into the sample hole. Start timer and wait for result.
-Interpret the result in 5-10 minutes. Result after 15 minutes is considered as invalid
Interpretation Of Result
- Positive (+): The presence of both "C" line and zone "T" line, no matter T line is clear or vague.
- Negative (-): Only clear C line appear. No T line.
- Invalid: No colored line appears in C zone. No matter if T line appears.
Certification
Company Profile
Hangzhou Evegen Biotech Co., Ltd. has a top colloidal gold product research and development team, strong technical strength, product quality ahead of similar products at home and abroad, after several times with the domestic and foreign well-known veterinary diagnostic reagents team cooperation, development and launch a series of broad market potential of livestock and poultry and pets with rapid diagnostic test paper products.
With the positive attitude and concept of win-win, the company develops more and better products for the international veterinary rapid diagnosis field. We are willing to contribute our youth and wisdom to the agriculture and animal disease detection industry. We are willing to join hands with those who are interested in creating the spring of rapid diagnosis of animal diseases in China!
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